Composite
C9

Part:BBa_K1366108:Design

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)


Apoptin and survivin siRNA production (C9)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1407
    Illegal BsaI site found at 2402


Design Notes

The construct structure is as follows: CMV // Apoptin // terminator // hTERT // siRNA // terminator


Source

The apoptin DNA sequence was retrieved from NCBI with the accession number of NC_001427.1. The siRNA was designed by us. And the rest of the parts were from the registry.

References